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The samples were subsequently reduced with 4 mM DTT for 30 min at room temperature with shaking at 1,000 rpm, followed by alkylation with 10 mM iodoacetamide for 45 min in the dark at room temperature while shaking at 1,000 rpm

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Each vial contains 10mg of lyophilized Cagrilintide in a sealed 3mL glass vial, verified through Mile High Compounds three-tier quality protocol : 7x independent third-party analytical testing, 4-vial batch conformity verification, and comprehensive heavy metal screening