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The establishment of stable cell lines expressing LCRMP-1 Plasmid pCMV-Tag2A- LCRMP-1 (3.5 g) was transfected into CL1-0 cells at 70% confluency using 20 U of Lipofectamine reagent (Invitrogen, #18324012) in a total volume of 1 ml of Opto-MEM (Gibco, #31985070)

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