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Notably, compared to epithelial liver cancer lines, HNF4-negative mesenchymal liver cancer lines also showed increased resistance to cell death induced by sorafenib when cultured in complete medium, and methionine/cystine restriction failed to enhance the effect of sorafenib in these cells (Fig

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Once they are opened they can be stored either in the refrigerator or at room temperature (59F to 86F) for up to 28 days