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Measurement of Cytokine Levels Cells seeded into sterile 25 cm 2 culture flasks (Corning) at 1.35 10 6 cells/flask were incubated to 80% confluence overnight, then washed with PBS and pretreated with control, NAC-containing or L-NAC-containing media (5.0 mM for 4 h) followed by challenge with control or PQ-containing media (0.25 or 1.0 mM for 4 h)

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