In multicellular organisms, Gsts display functional specificity to organs and tissues, underlying their necessity in growth and development 48,49,50,51
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This is consistent with the previously reported titration of GSSG during the reductive half-reaction of the U597C enzyme for which minimal kinetic changes were detected (Figure 10 reflects, this second reoxidation phase (with an estimated rate constant of 0.02 s 1 ) has been tentatively assigned to a conformational change with the movement of the reduced C596-C597 disulfide
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Gioiello, A., Piccinno, A., Lozza, A