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In a neonatal rat hypoxicischemic brain injury (HIBI) model, hydrogen significantly reduced the Bax/Bcl-2 ratio by up-regulating the expression of the anti-apoptotic protein Bcl-2, while down-regulating the expression of the pro-apoptotic protein Bax and the apoptosis-executing enzyme caspase-3, leading to a reduction in apoptosis (Wang et al., 2020)

High-performance target: Below 0.5 mg/L

Cell culture If not stated otherwise, cells were cultivated in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific) at 37 C with 5% CO 2 in a water saturated steam atmosphere