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[18] suggested that although GLP-1 RAs were related to a transient worsening of DR because of the rapid improvement in glycemic control, 71% of diabetes patients showed improvement of DR after continuing GLP-1 RAs treatment

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Cells isolation from freshly donated peripheral blood was done using Ficoll-paque plus density gradient centrifugation (Amersham Biosciences, Little Chalfont, UK)

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