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Mating in ragworms, Platynereis dumerilii , only occurs after the individuals have undergone a metamorphosis into a reproductive form that has only one purposethe release of gametes after which it dies (zpolat et al., 2021)

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Youll need insulin syringes (typically 0.5 mL or 1 mL capacity), alcohol pads for cleaning injection sites, a sharps container for safe needle disposal, and refrigeration for medication storage

Islets were then dispersed into single cells with calcium-free Hanks solution and used after incubation for another 24 hours in RPMI media containing either 5.6 mM glucose or 25 mM glucose