Analysis For the determination of free carnitine (FC) content, 5 L of the sample (plasma, muscle homogenate, and calibration points) was transferred into a 1.5 mL test tube, and then 200 L of acetonitrile containing the internal standard was added for protein precipitation
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Following the end of the sensation and motor studies, the rats were euthanized and both the left and right gastrocnemius muscles were dissected and weighed in order to compare the muscle mass of the operated limb (right) versus the non-operated limb (left) [2]
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