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Paracetamol is further metabolized by glucuronide conjugation and sulfate conjugation, which are non-toxic and easily excreted from the urine

Hemolysis Assay Following drug incubation, cells were spun down at 200 g for 5 min, the supernatant was collected, and absorbance was read at 405 nm on a CLARIOstar microplate reader

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When a toxic dose of paracetamol is ingested, the normal glucuronide pathway is saturated and large amounts of NAPQI are produced

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