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Several lines of evidence suggest that metformin improves intestinal microflora composition and intestinal barrier dysfunction (4345), suggesting that this pathway might be essential for metformin against NALFD

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Measurement of the TNF- and IL-1 by ELISA The TNF- and IL-1 levels in the perihematomal were determined using ELISA kits (USCN Life Science Inc., Wuhan, China) according to the manufacturers instructions

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